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KMID : 0861020170320060001
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Korea Journal of Herbology
2017 Volume.32 No. 6 p.1 ~ p.7
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Evaluation of Antioxidant and Anti-diabetic Effects of Sappan Lignum by Extraction Method
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Hong Young-Ju
Jeong Gyeong-Han
Jeong Yun-Hee
Kim Tae-Hoon
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Abstract
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Objectives : The heartwood of Sappan Lignum has been used since ancient times as an ingredient in folk medicines against anti-bacterial and anti-anemia purposes. Many bioactive constituents have been derived from this biomass such as chalcones and homoisoflavonoids. In the current investigation, the antioxidant and anti-diabetic properties using DPPH and ABTS+ radicals scavenging, α-glucosidase, and advanced glycation end products (AGEs) inhibition assays were evaluated by different extraction methods of Sappan Lignum.
Methods : In our continuing investigation for bioactive natural ingredients, the antioxidant and α-glucosidase inhibitory properties of Sappan Lignum extracts were prepared from different extraction methods and the biological efficacies were investigated in vitro. The antioxidant properties were evaluated employing radical scavenging assays using 1,1- diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) radicals. In addition, the anti-diabetic effects of Sappan Lignum extracts were tested via α-glucosidase and AGEs formation inhibitory assay. The total phenolic contents were determined using a spectrophotometric method.
Results : All the tested samples showed dose-dependent radical scavenging and α-glucosidase inhibitory activities. Among the tested extracts, the 80% methanolic extract of Sappan Lignum was showed the most potent activity with an IC50 value of 82.3±1.7 ㎍/㎖ against DPPH radical scavenging assay. While, ABTS+ radical scavenging activity of 80% methanolic extract was higher than those of other extracts. Also, α-glucosidase inhibitory and AGEs formation effects of each extacts and total phenolic contents were evaluated.
Conclusions : These results suggested that Sappan Lignum can be considered as a new effective source of natural antioxidant and anti-diabetic materials.
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KEYWORD
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Sappan Lignum, DPPH, ABTS+, α-glucosidase, advanced glycation end products (AGEs)
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